Eric Clapton said he will not perform in UK venues that require proof of vaccination for entry.
The English guitarist and singer announced the decision as the UK government eased social distancing restrictions this week.
With music venues and nightclubs functioning at full capacity, authorities are encouraging venue operators to use the National Health Services' Covid Pass to allow guests entry.
The online pass indicates if a person is fully vaccinated and the dates of their recent Covid tests.
Clapton said: "I wish to say that I will not perform on any stage where there is a discriminated audience present."
"Unless there is provision made for all people to attend, I reserve the right to cancel the show."
The news does not currently threaten his two concerts in London scheduled for next year.
Clapton is due to play at the Royal Albert Hall on Saturday, May 7 and Sunday, May 8, 2022, and for the time being, the venue does not require proof of vaccination for entry.
Tickets for both shows remain on sale online.
Clapton will embark on a US concert tour in September as the country's live music industry slowly re-emerges amid the pandemic.
That re-opening has not been a smooth process, however, with venues, due to a mix of regulations imposed by local authorities, artists and promoters, following different health and safety regulations.
Clapton's swing through the US, which includes cities Austin, Tampa and New Orleans will be in venues were no proof of vaccination is required.
Meanwhile, in other music touring news, Bruce Springsteen is in the midst of a 14-show residency at the St James Theatre on Broadway in New York City.
Running until Saturday, September 4, ticketholders have to prove they have received one of three Covid-19 vaccines approved for emergency use by the US Food and Drug Administration. These are Pfizer-BioNTech, Moderna and Johnson & Johnson.
The Foo Fighters are also on tour performing several shows in the US for vaccinated audiences, including in front of a 20,000 strong crowd at New York's Madison Square Garden last month.