Supporters will be allowed into the ground to watch September's Uefa Super Cup clash between Bayern Munich and Sevilla in Budapest.
Uefa announced on Tuesday that 30 per cent capacity at the Puskas Arena can be taken up by fans as Champions League winners Bayern taken on the Europa League victors from Spain on September 24.
The stadium has a 67,000 capacity which means there could be 30,000 fans at the game.
Uefa will use the game to study the impact of spectators as part of their Return to Play protocol.
However, European football's governing body also said all other matches will continue to be played behind closed doors.
The 2019-20 campaign has only just been completed after the coronavirus crisis caused a three-month delay across Europe's major leagues between March and June.
Premier League, Bundesliga, Serie A and La Liga games were all played in front of empty stadiums – although 5,000 people per match were allowed to watch their teams from the stands in the recently re-started French Ligue 1.
On Tuesday, though, French club Marseille announced that three more people have tested positive for the coronavirus, following five players last week.
Sseveral other Ligue 1 clubs have been affected by the virus, including Montpellier, Lens, Nice, Angers, Monaco and Brest.
"While it has been important to show that football can carry on in difficult times, without fans, the game has lost something of its character," said Uefa president Aleksander Ceferin.
"We hope to use the Uefa Super Cup in Budapest as a pilot that will begin to see the return of fans to our matches.
"We are working closely with the Hungarian federation and its government to implement measures to ensure the health of all those attending and participating in the game. We will not take risks with people's safety."
Cerfin said at the weekend that the single-game knockout format used in the Champions League this year from the quarterfinals onwards could be revisited in the future.
The format was changed in order to allow the competition to be completed in a shorter time span after the coronavirus pandemic halted play for several months across Europe in March.