The US said it was imposing sanctions on a "wide range of terrorists and their supporters, including the Palestinian group Hamas and Iran's Islamic Revolutionary Guard Corps, on Tuesday, the day before the 18th anniversary of the September 11 attacks.
The sanctions were imposed on 15 leaders, people and entities affiliated with the two groups and Al Qaeda, the US Treasury Department said.
The sanctions follow a counter-terrorism executive order by Donald Trump.
The order "enhances the authorities we use to target the finances of terror groups and their leaders to ensure they are as robust as possible,” said Treasury Secretary Steven Mnuchin, standing alongside Secretary of State Mike Pompeo.
Newly sacked US National Security Adviser John Bolton was not present.
Among those sanctioned are Muhammad Izad, a leader with the IRGC based in Lebanon, who is identified as the Palestinian office chief for IRGC Quds Force's Lebanon Corp.
Also under sanctions is Zaher Jabarin, based in Turkey, who is the head of Hamas’s finance office, the US said.
“In his capacity as head of Hamas’s finance department, Jabarin was focused on developing a financial network in Turkey that would allow Hamas to raise, invest, and launder money prior to transferring it to Gaza and the West Bank,” the Treasury said.
Also on the list is the company Redin Exchange, which the department said provided money to Hamas’ militant arm, the Ezzedine Al Qassam Brigades.
Redin’s leaders, Marwan Al Rawi and Ismael Tash, were designated individually. And Mr Tash owns Smart Ithalat Ihracat Dis Ticaret Ltd Sirketi, which was designated. Both companies are based in Istanbul.
Other companies sanctioned include the Saksouk Company for Exchange and Money Transfer, Al Haram Foreign Exchange and Al Khalidi Exchange, which were accused of supporting ISIS.
"Al Khalidi has materially assisted, sponsored, or provided financial, material, or technological support for, or goods or services to, ISIS," Treasury said.