Abu Dhabi-based private-sector employees working in "vital sectors and service industries" must undergo a PCR test every two weeks.
The compulsory tests will be free of charge, authorities said.
The committee said the decision would enhance precautionary measures as part of efforts to curb the spread of Covid-19 and protect public health.
Authorities have not yet specified which sectors and service industries the rule applies to.
But the news came as unvaccinated employees working in five types of businesses must take a PCR test every 14 days, under new federal rules.
The measure, which is effective as of March 28, applies to anyone employed by hotels, restaurants, transportation companies, laundries, beauty salons and hairdressers.
It is part of plans to “limit the spread of the virus, preserve the health of employees and keep work environments safe,” according to a briefing note issued by The Ministry of Human Resources and Emiratisation.
The decision was made jointly with the National Emergency Crisis and Management Authority, it said.
The number of daily tests has grown in recent weeks.
Many employees are tested routinely and regularly, in some cases as often as every seven days in Abu Dhabi.