Venus Williams and Kim Clijsters both fell at the first hurdle as seeded players dominated the women's draw for a second straight day at the US Open.
Hours after younger sister Serena secured her place in the second round, Venus, 40, was back in the spotlight in what was an incredible 22nd US Open appearance.
But Venus, who reached the final of the US Open on her debut at the tournament in 1997, was beaten in the first round for the first time in her glittering career by Karolina Muchova of the Czech Republic.
Muchova, the 20th seed, had too much poise for Williams en route to a 6-3, 7-5 win.
Defeat to the 20th seed means Venus has failed to go beyond the third round of a grand slam since 2017.
Despite her 6-3, 7-5 setback, the seven-time major winner insists that she plans to continue playing, starting with this month's French Open at Roland Garros.
"I love this game," Williams said. "I'm good at what I do. It's easy to stay motivated and excited about doing something so amazing. Not many people get to do this.
"But I look forward to a few days off. It's been a lot of work. Looking forward to just having a little bit of a rest before the clay courts."
Belgium's Clijsters meanwhile, making the second comeback of her career and her first appearance at a Grand Slam since 2012, raised hopes of an upset after taking the first set against Russia's Ekaterina Alexandrova.
The 37-year-old three-time US Open champion ran out of steam though as Alexandrova, seeded 21, completed a 3-6, 7-5, 6-1 win.
This year's women's field in New York is more open than ever with world number one Ashleigh Barty, the second-ranked Simona Halep and defending champion Bianca Andreescu all electing to skip the tournament.
One of the leading contenders for the title, second seed Sofia Kenin of the United States, got off the mark with a 6-2, 6-2 win over unseeded Yanina Wickmayer of Belgium.
The 21-year-old, who won a maiden Grand Slam title at this year's Australian Open, later revealed she had cried to herself before the match in order to alleviate stress.
"Crying. That's what I did. I mean, I had to let it out," Kenin said. "It's fine. It worked."
Kenin will play Canada's Leylah Fernandez in the second round. Fernandez opened her campaign with a 6-4, 7-5, win over Vera Zvonareva on Tuesday.
Ninth seed Jo Konta meanwhile won the 'Battle of Britain' against compatriot Heather Watson, winning 7-6, 6-1.
"There's always going to be rivalry between compatriots," Konta said. "It's always going to be that extra stressful.
"There's always going to be an element of people having more judgments and more of an opinion on the outside at home on a match like today," added Konta, who will play Romania's Sorana Cirstea in the second round.
MEYDAN%20RACECARD
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MATCH INFO
Manchester United v Everton
Where: Old Trafford, Manchester
When: Sunday, kick-off 7pm (UAE)
How to watch: Live on BeIN Sports 11HD
GRAN%20TURISMO
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Results
STAGE
1 . Filippo Ganna (Ineos) - 0:13:56
2. Stefan Bissegger (Education-Nippo) - 0:00:14
3. Mikkel Bjerg (UAE Team Emirates) - 0:00:21
4. Tadej Pogacar (UAE Team Emirates) - 0:00:24
5. Luis Leon Sanchez (Astana) - 0:00:30
GENERAL CLASSIFICATION
1. Tadej Pogacar (UAE Team Emirates) - 4:00:05
2. Joao Almeida (QuickStep) - 0:00:05
3. Mattia Cattaneo (QuickStep) - 0:00:18
4. Chris Harper (Jumbo-Visma) - 0:00:33
5. Adam Yates (Ineos) - 0:00:39
SUZUME
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SPEC%20SHEET%3A%20APPLE%20M3%20MACBOOK%20AIR%20(13%22)
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The five pillars of Islam
The Sand Castle
Director: Matty Brown
Stars: Nadine Labaki, Ziad Bakri, Zain Al Rafeea, Riman Al Rafeea
Rating: 2.5/5
BUNDESLIGA FIXTURES
Saturday
Borussia Dortmund v Eintracht Frankfurt (5.30pm kick-off UAE)
Bayer Leverkusen v Schalke (5.30pm)
Wolfsburg v Cologne (5.30pm)
Mainz v Arminia Bielefeld (5.30pm)
Augsburg v Hoffenheim (5.30pm)
RB Leipzig v Bayern Munich (8.30pm)
Borussia Monchengladbach v Freiburg (10.30pm)
Sunday
VfB Stuttgart v Werder Bremen (5.30pm)
Union Berlin v Hertha Berlin (8pm)
Scores
Wales 74-24 Tonga
England 35-15 Japan
Italy 7-26 Australia
Company%20Profile
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RESULTS
Dubai Kahayla Classic – Group 1 (PA) $750,000 (Dirt) 2,000m
Winner: Deryan, Ioritz Mendizabal (jockey), Didier Guillemin (trainer).
Godolphin Mile – Group 2 (TB) $750,000 (D) 1,600m
Winner: Secret Ambition, Tadhg O’Shea, Satish Seemar
Dubai Gold Cup – Group 2 (TB) $750,000 (Turf) 3,200m
Winner: Subjectivist, Joe Fanning, Mark Johnston
Al Quoz Sprint – Group 1 (TB) $1million (T) 1,200m
Winner: Extravagant Kid, Ryan Moore, Brendan Walsh
UAE Derby – Group 2 (TB) $750,000 (D) 1,900m
Winner: Rebel’s Romance, William Buick, Charlie Appleby
Dubai Golden Shaheen – Group 1 (TB) $1.5million (D) 1,200m
Winner: Zenden, Antonio Fresu, Carlos David
Dubai Turf – Group 1 (TB) $4million (T) 1,800m
Winner: Lord North, Frankie Dettori, John Gosden
Dubai Sheema Classic – Group 1 (TB) $5million (T) 2,410m
Winner: Mishriff, John Egan, John Gosden
Company%20Profile
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SPEC%20SHEET%3A%20NOTHING%20PHONE%20(2a)
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Nick's journey in numbers
Countries so far: 85
Flights: 149
Steps: 3.78 million
Calories: 220,000
Floors climbed: 2,000
Donations: GPB37,300
Prostate checks: 5
Blisters: 15
Bumps on the head: 2
Dog bites: 1
More from Rashmee Roshan Lall
THURSDAY'S FIXTURES
4pm Maratha Arabians v Northern Warriors
6.15pm Deccan Gladiators v Pune Devils
8.30pm Delhi Bulls v Bangla Tigers
India squad
Virat Kohli (captain), Rohit Sharma, Mayank Agarwal, K.L. Rahul, Shreyas Iyer, Manish Pandey, Rishabh Pant, Shivam Dube, Kedar Jadhav, Ravindra Jadeja, Yuzvendra Chahal, Kuldeep Yadav, Deepak Chahar, Mohammed Shami, Shardul Thakur.
UJDA CHAMAN
Produced: Panorama Studios International
Directed: Abhishek Pathak
Cast: Sunny Singh, Maanvi Gagroo, Grusha Kapoor, Saurabh Shukla
Rating: 3.5 /5 stars
COMPANY PROFILE
Name: Rain Management
Year started: 2017
Based: Bahrain
Employees: 100-120
Amount raised: $2.5m from BitMex Ventures and Blockwater. Another $6m raised from MEVP, Coinbase, Vision Ventures, CMT, Jimco and DIFC Fintech Fund
The Kites
Romain Gary
Penguin Modern Classics