England have dropped Phil Foden and Mason Greenwood for their friendly with Wales and Nations League matches against Belgium and Denmark this month.
Manager Gareth Southgate has named Harry Maguire in his 30-man squad.
Manchester City midfielder Foden and Manchester United forward Greenwood made their senior England debuts against Iceland before being fined by local police and sent home for breaching Covid-19 regulations.
Both players had issued apologies in the aftermath of the incident but Southgate had said they would have to regain his trust before they can be considered for selection.
Southgate added that Foden and Greenwood would be back in contention come his next squad.
He said: "We have to send a message to all our players in our teams at every age level that that sort of thing isn't how we do things with England.
"Then we've got to rehabilitate these two boys. Young people will make mistakes. These lads have suffered enough now.
"I think they need this period of reflection but that gives them the opportunity to get back on their feet with their clubs, enjoy their football again.
"I can speak to them after this camp and it will be just be about their form then."
Maguire returns after he was withdrawn for the Nations League matches against Iceland and Denmark following his arrest and trial in Greece during the close season.
A Greek court found Maguire guilty of repeated bodily harm, attempted bribery, violence against public employees and insult following an incident on the island of Mykonos.
The 27-year-old was handed a suspended prison sentence of 21 months and 10 days but the United captain has been granted a full retrial after appealing the convictions.
He has since played in United's two opening Premier League games, as well as the Carabao Cup win at Luton.
Southgate's England play Wales on October 8, before two more home games against top-ranked Belgium on October 11 and Denmark on Octtober 14.
There were first call-ups for Everton striker Dominic Calvert-Lewin, who has scored eight goals in the opening five games of the season, as well as Leicester's Harvey Barnes and Bukayo Saka of Arsenal.
Welcome to the #ThreeLions, lads! 👊 pic.twitter.com/DKMjUnY54T
— England (@England) October 1, 2020
Southgate believes that in-form Calvert-Lewin has made the key improvement vital to any striker's game – scoring goals
“He’s been in the pathway through the U21s, had a good season last season," said Southgate.
“A lot of Dominic’s game I’ve liked for a long time, what he’s adding now is the goals that haven’t always been there and in the last 18 months or so he has really improved in that area.
“He’s been a big part of Everton’s starting the season as well as they have and I’m very pleased to be able to call him up for the first time.”
The Everton striker expressed his pride at receiving his first call-up. "I'm a very, very proud man to get this first England call," Calvert-Lewin told Everton's club website.
"I just phoned my mum and my dad, and my mum burst into tears. It's something we've spoken about since I was a young boy. To get here now and get the call-up is a dream come true. I'm very happy."
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England player ratings for goalless draw with Denmark
