The Abu Dhabi Department of Economic Development has closed 325 businesses in Abu Dhabi, Al Ain and Al Dhafra, after staff tested positive for the coronavirus.
Another 29 businesses were shut for failing to abide by Covid-19 safety rules.
The department urged business owners to follow all precautionary measures to help curb the infection and ensure the safety of their staff.
In Dubai, authorities temporarily closed 32 food outlets and issued warnings to a further 472 for flouting Covid-19 rules this year.
Dubai Municipality said its inspectors have carried out 5,841 visits in 2021 to establishments such as restaurants, cafes, supermarkets and grocery stores, to ensure they are following safety measures.
Traders are allowed to operate during the pandemic, but must comply with coronavirus regulations governing hygiene, wearing face masks and social distancing.
Coronavirus cases and deaths have increased in the UAE since the start of the year.
The country has recorded 191 deaths in February, so far.
Officials have taken steps to drive down infections.
Schools in Sharjah, Ajman, Ras Al Khaimah and Umm Al Quwain switched to online learning while capacity at public venues such as malls, cinemas and hotels was cut in all parts of the country.
Inspections in malls and business establishments are being regularly carried out to ensure compliance with the rules.