Covid-19 test providers in Abu Dhabi face being fined or banned from offering PCR services to the public if they fail to abide by a fixed Dh65 ($17.70) price for the nasal swab test.
The Department of Health in Abu Dhabi said centres would face tough action if they flouted rules set down by authorities.
In June, a healthcare centre was fined for failing to provide PCR tests at the mandated cost.
The directive covers swab collection, testing and reporting of results.
"The fixed price for the PCR test is applied to both regular and urgent services," the Department of Health said.
"If any healthcare provider is not in compliance with this protocol, they will be terminated from providing PCR testing services and fines will be issued as per applicable legislations."
Health officials in the capital reduced the cost of a PCR test to Dh65 at healthcare clinics and screening centres in March.
The cost of PCR testing has been gradually reduced during the pandemic as part of a mass screening drive.
The nasal swab test was typically priced at Dh370 in the early months of the outbreak, but the cost was reduced to Dh180 in Abu Dhabi before being again cut to Dh85 and then Dh65.
The health authority called on members of the community to inform it of any breaches of the pricing regulation by calling (02) 419 3845 or emailing Healthsystemfinancing@doh.gov.ae.
More than 67.6 million PCR tests have been carried out across the country to date.
The test, regarded as the gold standard for Covid-19 tests, is a requirement for international travel, as well entry to Abu Dhabi from other Emirates.
Many employees, particularly those in service industries, must take regular PCR tests if they are not vaccinated against Covid-19.